dopc dope dopg (Avanti Polar)
Structured Review

Dopc Dope Dopg, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 96/100, based on 592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dopc dope dopg/product/Avanti Polar
Average 96 stars, based on 592 article reviews
Images
1) Product Images from "The membrane transporter lactose permease increases lipid bilayer bending rigidity"
Article Title: The membrane transporter lactose permease increases lipid bilayer bending rigidity
Journal: Biophysical Journal
doi: 10.1016/j.bpj.2021.06.038
Figure Legend Snippet: ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of DOPG mol% increase for ( a ) DOPC/DOPE (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.
Techniques Used: Fluorescence, Microscopy
Figure Legend Snippet: ( Top ) Z-projections of Atto488-LacY GUVs showing protein increase. ( Bottom ) LacY quantification in Atto488-LacY LUVs and Atto488-LacY GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%). The protein/lipid ratio given in the first row is that calculated from the initial concentrations used in reconstitution, and the remaining rows are the measured concentrations in the LacY LUVs and LacY GUVs. ∗ Quantification by the biochemical Markwell-Lowry assay in LUVs. ∗∗ Quantification by confocal fluorescence microscopy in GUVs. Number of Atto488-LacY ± standard deviation is given. n, number of GUVs measured.
Techniques Used: Lowry Assay, Fluorescence, Microscopy, Standard Deviation
Figure Legend Snippet: Bending rigidities for GUVs with the lipid composition DOPC/DOPE/DOPG as function of the detergent removal method
Techniques Used: Negative Control
Figure Legend Snippet: LacY crowding in GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%) and (20:20:60 mol%) as a function of the bending rigidity parameter. Bending rigidity values in circles and squares correspond to 50 mM NaPhos and triangles to PBS. Error bar = mean ± SE.
Techniques Used: