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dopc dope dopg  (Avanti Polar)


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    Structured Review

    Avanti Polar dopc dope dopg
    ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of <t>DOPG</t> mol% increase for ( a ) <t>DOPC/DOPE</t> (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.
    Dopc Dope Dopg, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 96/100, based on 592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The membrane transporter lactose permease increases lipid bilayer bending rigidity"

    Article Title: The membrane transporter lactose permease increases lipid bilayer bending rigidity

    Journal: Biophysical Journal

    doi: 10.1016/j.bpj.2021.06.038

    ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of DOPG mol% increase for ( a ) DOPC/DOPE (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.
    Figure Legend Snippet: ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of DOPG mol% increase for ( a ) DOPC/DOPE (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.

    Techniques Used: Fluorescence, Microscopy

    ( Top ) Z-projections of Atto488-LacY GUVs showing protein increase. ( Bottom ) LacY quantification in Atto488-LacY LUVs and Atto488-LacY GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%). The protein/lipid ratio given in the first row is that calculated from the initial concentrations used in reconstitution, and the remaining rows are the measured concentrations in the LacY LUVs and LacY GUVs. ∗ Quantification by the biochemical Markwell-Lowry assay in LUVs. ∗∗ Quantification by confocal fluorescence microscopy in GUVs. Number of Atto488-LacY ± standard deviation is given. n, number of GUVs measured.
    Figure Legend Snippet: ( Top ) Z-projections of Atto488-LacY GUVs showing protein increase. ( Bottom ) LacY quantification in Atto488-LacY LUVs and Atto488-LacY GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%). The protein/lipid ratio given in the first row is that calculated from the initial concentrations used in reconstitution, and the remaining rows are the measured concentrations in the LacY LUVs and LacY GUVs. ∗ Quantification by the biochemical Markwell-Lowry assay in LUVs. ∗∗ Quantification by confocal fluorescence microscopy in GUVs. Number of Atto488-LacY ± standard deviation is given. n, number of GUVs measured.

    Techniques Used: Lowry Assay, Fluorescence, Microscopy, Standard Deviation

    Bending rigidities for GUVs with the lipid composition  DOPC/DOPE/DOPG  as function of the detergent removal method
    Figure Legend Snippet: Bending rigidities for GUVs with the lipid composition DOPC/DOPE/DOPG as function of the detergent removal method

    Techniques Used: Negative Control

    LacY crowding in GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%) and (20:20:60 mol%) as a function of the bending rigidity parameter. Bending rigidity values in circles and squares correspond to 50 mM NaPhos and triangles to PBS. Error bar = mean ± SE.
    Figure Legend Snippet: LacY crowding in GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%) and (20:20:60 mol%) as a function of the bending rigidity parameter. Bending rigidity values in circles and squares correspond to 50 mM NaPhos and triangles to PBS. Error bar = mean ± SE.

    Techniques Used:



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    ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of <t>DOPG</t> mol% increase for ( a ) <t>DOPC/DOPE</t> (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.
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    ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of <t>DOPG</t> mol% increase for ( a ) <t>DOPC/DOPE</t> (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.
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    Image Search Results


    ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of DOPG mol% increase for ( a ) DOPC/DOPE (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.

    Journal: Biophysical Journal

    Article Title: The membrane transporter lactose permease increases lipid bilayer bending rigidity

    doi: 10.1016/j.bpj.2021.06.038

    Figure Lengend Snippet: ( Top panels ) Z-projection images from confocal fluorescence microscopy. From left to right are shown Atto488-LacY GUVs as a function of DOPG mol% increase for ( a ) DOPC/DOPE (50:50 mol%), ( b ) DOPC/DOPE/DOPG (40:40:20 mol%), and ( c ) DOPC/DOPE/DOPG (20:20:60 mol%) lipid mixtures. The dotted rectangle in the images indicates the GUV cross section used to determine Atto488 fluorescence intensity across the GUVs shown in the lower graphs. Two intensity maxima (in gray level arbitrary units) in each plot correspond to the fluorescence of Atto488-LacY in the GUV lipid bilayer for one of the confocal slides used for the Z-projection images.

    Article Snippet: Large unilamellar vesicles (LUVs) were formed by extrusion with the lipid mixtures 1,2-dioleoyl-glycero-3-phosphocholine (DOPC)/1,2-dioleoyl-glycero-3-phosphoethanolamine (DOPE) (50:50 mol%), DOPC/DOPE/1,2-dioleoyl-glycero-3phosphoglycerol (DOPG) (40:40:20 mol%), and DOPC/DOPE/DOPG (20:20:60 mol%) (Avanti Polar Lipids, Alabaster, AL).

    Techniques: Fluorescence, Microscopy

    ( Top ) Z-projections of Atto488-LacY GUVs showing protein increase. ( Bottom ) LacY quantification in Atto488-LacY LUVs and Atto488-LacY GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%). The protein/lipid ratio given in the first row is that calculated from the initial concentrations used in reconstitution, and the remaining rows are the measured concentrations in the LacY LUVs and LacY GUVs. ∗ Quantification by the biochemical Markwell-Lowry assay in LUVs. ∗∗ Quantification by confocal fluorescence microscopy in GUVs. Number of Atto488-LacY ± standard deviation is given. n, number of GUVs measured.

    Journal: Biophysical Journal

    Article Title: The membrane transporter lactose permease increases lipid bilayer bending rigidity

    doi: 10.1016/j.bpj.2021.06.038

    Figure Lengend Snippet: ( Top ) Z-projections of Atto488-LacY GUVs showing protein increase. ( Bottom ) LacY quantification in Atto488-LacY LUVs and Atto488-LacY GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%). The protein/lipid ratio given in the first row is that calculated from the initial concentrations used in reconstitution, and the remaining rows are the measured concentrations in the LacY LUVs and LacY GUVs. ∗ Quantification by the biochemical Markwell-Lowry assay in LUVs. ∗∗ Quantification by confocal fluorescence microscopy in GUVs. Number of Atto488-LacY ± standard deviation is given. n, number of GUVs measured.

    Article Snippet: Large unilamellar vesicles (LUVs) were formed by extrusion with the lipid mixtures 1,2-dioleoyl-glycero-3-phosphocholine (DOPC)/1,2-dioleoyl-glycero-3-phosphoethanolamine (DOPE) (50:50 mol%), DOPC/DOPE/1,2-dioleoyl-glycero-3phosphoglycerol (DOPG) (40:40:20 mol%), and DOPC/DOPE/DOPG (20:20:60 mol%) (Avanti Polar Lipids, Alabaster, AL).

    Techniques: Lowry Assay, Fluorescence, Microscopy, Standard Deviation

    Bending rigidities for GUVs with the lipid composition  DOPC/DOPE/DOPG  as function of the detergent removal method

    Journal: Biophysical Journal

    Article Title: The membrane transporter lactose permease increases lipid bilayer bending rigidity

    doi: 10.1016/j.bpj.2021.06.038

    Figure Lengend Snippet: Bending rigidities for GUVs with the lipid composition DOPC/DOPE/DOPG as function of the detergent removal method

    Article Snippet: Large unilamellar vesicles (LUVs) were formed by extrusion with the lipid mixtures 1,2-dioleoyl-glycero-3-phosphocholine (DOPC)/1,2-dioleoyl-glycero-3-phosphoethanolamine (DOPE) (50:50 mol%), DOPC/DOPE/1,2-dioleoyl-glycero-3phosphoglycerol (DOPG) (40:40:20 mol%), and DOPC/DOPE/DOPG (20:20:60 mol%) (Avanti Polar Lipids, Alabaster, AL).

    Techniques: Negative Control

    LacY crowding in GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%) and (20:20:60 mol%) as a function of the bending rigidity parameter. Bending rigidity values in circles and squares correspond to 50 mM NaPhos and triangles to PBS. Error bar = mean ± SE.

    Journal: Biophysical Journal

    Article Title: The membrane transporter lactose permease increases lipid bilayer bending rigidity

    doi: 10.1016/j.bpj.2021.06.038

    Figure Lengend Snippet: LacY crowding in GUVs with the lipid composition DOPC/DOPE/DOPG (40:40:20 mol%) and (20:20:60 mol%) as a function of the bending rigidity parameter. Bending rigidity values in circles and squares correspond to 50 mM NaPhos and triangles to PBS. Error bar = mean ± SE.

    Article Snippet: Large unilamellar vesicles (LUVs) were formed by extrusion with the lipid mixtures 1,2-dioleoyl-glycero-3-phosphocholine (DOPC)/1,2-dioleoyl-glycero-3-phosphoethanolamine (DOPE) (50:50 mol%), DOPC/DOPE/1,2-dioleoyl-glycero-3phosphoglycerol (DOPG) (40:40:20 mol%), and DOPC/DOPE/DOPG (20:20:60 mol%) (Avanti Polar Lipids, Alabaster, AL).

    Techniques: